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Updated: Aug 14, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Published on: March 6, 2018
Stochastic reconstruction of particulate media from two-dimensional images
M S Talukdar1, O Torsaeter, M A Ioannidis
1Department of Petroleum Engineering and Applied Geophysics, Norwegian University of Science and Technology, Trondheim, Norway. mioannid@cape.uwaterloo.ca
Abstract:
In this contribution we address the problem of reconstructing particulate media from limited morphological information that may be readily extracted from 2D images of their microstructure. Sixty-five backscatter SEM images of the microstructure of a lightly consolidated pack of glass spheres are analyzed to determine morphological descriptors, such as the pore-pore autocorrelation function and pore and solid phase chord distributions. This information is then used to constrain the stochastic reconstruction of the glass sphere packing in two dimensions using a simulated annealing method. The results obtained demonstrate that the solid-phase chord distribution contains additional information that is critical for the reconstruction of the morphology of particulate media exhibiting short-range order. We further confirm this finding by successfully reconstructing the microstructure of a pack of irregular silica particles.

