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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Published on: October 23, 2014
Antisense-mediated knockdown of iNOS expression in the presence of cytokines
Karsten Hemmrich1, Christoph V Suschek, Victoria Kolb-Bachofen
1Department of Plastic Surgery, Hand Surgery, Burn Center, University Hospital of the Aachen, University of Technology, Aachen, Germany.
Abstract:
The impact of nitric oxide (NO) synthesized after activation by proinflammatory cytokines and/or bacterial products by an inducible NO synthase (iNOS) is still contradictory. Various methods to inhibit iNOS expression or activity have been established. A relatively new approach to inhibit iNOS-derived NO production is the antisense (AS) technique, which theoretically provides a specific and efficient method for inhibiting gene expression and function. This chapter focuses on the application of iNOS-specific AS-oligodeoxynucleotide (ODN) and highlights some of the pitfalls that must be considered to use this technique effectively.
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