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Related Experiment Videos

P2X receptors in mouse Leydig cells.

Luiz Artur Poletto Chaves1, Endrigo Piva Pontelli, Wamberto Antonio Varanda

  • 1Department of Physiology, School of Medicine of Ribeirão Preto/USP, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto/SP, Brazil.

American Journal of Physiology. Cell Physiology
|November 18, 2005
PubMed
Summary

This study investigated ATP-activated currents in mouse Leydig cells, identifying a P2X2-like receptor. The findings reveal its properties, including ion permeability and activation kinetics, crucial for understanding Leydig cell function.

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Area of Science:

  • Cellular electrophysiology
  • Purinergic signaling
  • Steroidogenesis

Background:

  • Leydig cells are crucial for testosterone production.
  • ATP signaling plays a role in regulating Leydig cell function.
  • The specific ATP receptors in Leydig cells remain incompletely characterized.

Purpose of the Study:

  • To characterize the ATP-activated currents in mouse Leydig cells.
  • To determine the properties and potential identity of the ATP receptor involved.
  • To elucidate the role of this receptor in Leydig cell physiology.

Main Methods:

  • Patch-clamp technique (whole-cell and outside-out configurations).
  • Application of various ATP analogs and receptor antagonists.
  • Concentration-response and current-voltage relationship analyses.

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  • Bi-ionic conditions to assess ion permeability.

    • Main Results:

    • ATP induced rapidly activating, slowly desensitizing currents with inward rectification.
    • The receptor showed specific agonist efficacy (ATP > ATPγS > 2-MeS-ATP) and was blocked by Suramin and PPADS.
    • The channel exhibited high Ca2+ permeability (P(Ca)/P(Na) = 5.32) and a chord conductance of 27 pS.
    • Current properties were pH-dependent, with maximal activity at pH 6.5.

    Conclusions:

    • Leydig cells express an ATP-activated ion channel.
    • The receptor's characteristics closely resemble the cloned homomeric P2X2 receptor.
    • These findings provide insights into purinergic modulation of Leydig cell function.