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Related Experiment Videos

Gap junctions modulate interkinetic nuclear movement in retinal progenitor cells.

Rachael A Pearson1, Nanna L Lüneborg, David L Becker

  • 1Department of Physiology, University College London, London WC1E 6BT, United Kingdom. rachael.pearson@ich.ucl.ac.uk

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|November 18, 2005
PubMed
Summary

Gap-junctional communication and calcium (Ca2+) activity regulate nuclear movement in retinal progenitor cells during development. Disrupting these processes slows cell cycle progression and nuclear migration.

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Area of Science:

  • Developmental Biology
  • Neuroscience
  • Cell Biology

Background:

  • Retinal progenitor cells divide to expand the progenitor pool during early development.
  • Interkinetic nuclear movement, a back-and-forth migration of nuclei, occurs during G1 and G2 phases of the cell cycle.
  • Nuclear division is restricted to the ventricular surface, making nuclear movement speed a potential cell cycle modulator.

Purpose of the Study:

  • To investigate the role of gap-junctional coupling and calcium activity in interkinetic nuclear movement of retinal progenitor cells.
  • To determine how these factors influence the rate and nature of nuclear migration during retinal development.

Main Methods:

  • Time-lapse confocal microscopy to observe nuclear movement and Ca2+ transients.
  • Buffering intracellular Ca2+ transients using BAPTA.

Related Experiment Videos

  • Inhibition of gap junctions using conventional blockers and dominant-negative connexin 43 (Cx43) constructs.
  • Application of Cx43-specific antisense oligodeoxynucleotides (asODNs) and the gap junction mimetic peptide Gap26.

    • Main Results:

    • Nuclear movement is often, but not always, associated with Ca2+ transients; buffering these transients slows movement.
    • Gap-junctional communication is essential for normal interkinetic nuclear movement in retinal progenitor cells.
    • Inhibition of gap junctions, Cx43, or hemichannels significantly slows nuclear migration.

    Conclusions:

    • Gap-junctional communication and associated Ca2+ signaling are critical regulators of interkinetic nuclear movement in developing retinal progenitor cells.
    • These findings highlight a novel mechanism linking cell-cell communication to cell cycle regulation during neurodevelopment.