Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Myeloperoxidase activity imaging using (67)Ga labeled substrate.

Manel Querol Sans1, John W Chen, Ralph Weissleder

  • 1Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA.

Molecular Imaging and Biology
|November 18, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering and Targeting the Schwannoma-Neuron-Macrophage Crosstalk for the Treatment of Schwannomatosis and Associated Pain.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Case 2-2026: A 63-Year-Old Man with Pulmonary Nodules, Liver Mass, and Vision Loss.

The New England journal of medicine·2026
Same author

High-speed wide-field fluorescence lifetime imaging for intraoperative tumor visualization and in vivo multiplexing.

Biomedical optics express·2025
Same author

AAV hamartin gene therapy in a stochastic, cerebral mouse model of tuberous sclerosis type 1.

Molecular therapy. Methods & clinical development·2025
Same author

Epidemiology and burden of adult chronic pancreatitis in South Australia: a 20-year data linkage study.

BMJ open·2025
Same author

A specific and adaptable approach to track CD206<sup>+</sup> macrophages by molecular MRI and fluorescence imaging.

Theranostics·2025
Same journal

Direct Androgen Receptor Antagonism Enhances Therapeutic PSMA Radioligand Uptake in Prostate Cancer Models.

Molecular imaging and biology·2026
Same journal

Advances in PD-L1 Targeted Molecular Imaging Radiotracers Research: From Preclinical Exploration to Clinical Application.

Molecular imaging and biology·2026
Same journal

Preclinical Evaluation of [<sup>18</sup>F]JNJ-1: A Novel Positron Emission Tomography Ligand Targeting AMPAR/TARP γ8.

Molecular imaging and biology·2026
Same journal

Probing Early Myocardial Remodeling in Response to a High-Fat Diet in Mice by Evaluation of Extracellular Volume Fraction Using 4D Retrospectively Gated Micro-CT Imaging.

Molecular imaging and biology·2026
Same journal

Molecular Imaging of Butyrylcholinesterase Associated with Amyloid-β Plaques Distinguishes 5XFAD from Wild-Type Mice: A Proof-of-Concept.

Molecular imaging and biology·2026
Same journal

Brain Amyloid Deposition Is Negatively Associated with Cardiac Amyloid Retention in Apo E4 Carriers: A Pilot Study.

Molecular imaging and biology·2026
See all related articles

This study demonstrates the feasibility of imaging myeloperoxidase (MPO) activity using a novel gallium-67 labeled radiotracer. The tracer allows for SPECT imaging of MPO in a preclinical model, showing increased uptake in MPO-rich tissues.

Area of Science:

  • Nuclear medicine
  • Radiopharmaceutical chemistry
  • Molecular imaging

Background:

  • Myeloperoxidase (MPO) is a key enzyme in inflammation and atherosclerosis.
  • Imaging MPO activity can provide insights into disease processes.

Purpose of the Study:

  • To assess the feasibility of single photon emission computed tomography (SPECT) imaging of MPO activity.
  • To utilize a novel (67)Ga-labeled radiotracer for MPO imaging in vivo.

Main Methods:

  • Synthesis of a novel (67)Ga-labeled radiotracer by conjugating desferrioxamine (DF) and hydroxyindolyl acetic acid.
  • In vitro characterization of the radiotracer's interaction with MPO.
  • In vivo SPECT/CT imaging and biodistribution studies in a mouse model with MPO-rich Matrigel implants.

Related Experiment Videos

Main Results:

  • Confirmed in vitro MPO-mediated oligomerization and cross-linking of the radiotracer.
  • Achieved a 2.7-fold increase in target-to-muscle ratio in MPO-containing implants.
  • Observed a 60% increase in radioactivity in MPO-containing implants compared to controls.

Conclusions:

  • The novel (67)Ga-labeled radiotracer enables imaging of MPO activity in a model system.
  • Accumulation is driven by size increase due to radiotracer interaction with MPO, altering pharmacokinetics.