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Determination of 4-aminopyridine in serum by solid-phase extraction and high-performance liquid chromatography
A van der Horst1, P N de Goede, H A van Diemen
1Department of Pharmacy, Free University Hospital, Amsterdam, Netherlands.
Journal of Chromatography
|February 7, 1992
Abstract:
An assay for the determination of 4-aminopyridine in serum has been developed using 3,4-diaminopyridine as internal standard and reversed-phase high-performance liquid chromatography with detection at 244 nm. A mobile phase of acetonitrile-methanol-ethanol-1% ammonium carbonate (75:10:10:5) provided excellent separation of both compounds. Samples were extracted on solid-phase columns. The linearity, precision, recovery and the limit of detection were all sufficient for the routine use of this assay in clinical studies of patients treated with 4-aminopyridine.