Related Experiment Video

Updated: Aug 14, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

ATP-dependent chromatin-remodeling complexes in DNA double-strand break repair: remodeling, pairing and (re)pairing

Jian Huang1, Bing Liang, Jiajing Qiu

  • 1Department of Oncological Science, Mount Sinai School of Medicine, New York, New York 10029, USA.

Cell Cycle (Georgetown, Tex.)
|November 19, 2005
PubMed

Insights

Eukaryotic cells repair thousands of daily DNA breaks using chromatin remodelers like RSC and Swi/Snf. These enzymes are crucial for homologous recombination repair and maintaining genomic stability, preventing cancer.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic cells face over 10,000 DNA lesions daily, necessitating robust repair mechanisms.
  • Defective DNA repair can result in chromosomal abnormalities and cancer.
  • Chromatin structure influences DNA damage repair, implicating chromatin-modifying and remodeling enzymes.

Purpose of the Study:

  • To explore the recruitment of ATP-dependent chromatin-remodeling complexes to DNA breaks.
  • To understand the extensive recruitment of remodelers to double-strand breaks (DSBs).
  • To investigate a potential link between RSC's role in sister chromatid cohesion and DSB repair.

Main Methods:

  • Discussion of existing research on chromatin remodelers in DNA repair.
  • Analysis of the recruitment mechanisms of enzymes like RSC and Swi/Snf to DNA damage sites.
  • Exploration of the functional interplay between chromatin remodeling, sister chromatid cohesion, and homologous recombination.

Main Results:

  • RSC and Swi/Snf ATP-dependent chromatin-remodeling complexes are involved in DSB repair via homologous recombination.
  • Multiple chromatin remodelers are recruited to sites of DSBs.
  • A functional connection between RSC's roles in sister chromatid cohesion and DSB repair is proposed.

Conclusions:

  • Chromatin remodelers play a significant role in the DNA damage response, particularly in homologous recombination.
  • Understanding the recruitment and function of these remodelers is key to comprehending genomic integrity maintenance.
  • Further research into the RSC-chromatin cohesion-DSB repair link may reveal novel therapeutic targets for cancer.

More Related Videos

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...