Humic material induces behavioral and global transcriptional responses in the nematode Caenorhabditis elegans

Humic materials are complex organic molecules constituting the most abundant source of natural organic matter (NOM) in freshwater and soil ecosystems. Recent advances have identified that they interfere with biological systems, via the induction of biotransformation enzymes, the inhibition of photosynthetic oxygen release (in freshwater plants), the production of internal oxidative stress, or through the feminization of fish and amphibians. The nematode model organism Caenorhabditis elegans was chosen to investigate whether a natural and a synthetic humic material induce (i) a behavioral attraction, (ii) the reproduction, and (iii) a response in whole genome transcriptional expression. The phenomenological attractant experiments provided evidence that both humic material sources attract the worm and exert distinct chemical cues. In the reproduction assay, only the highest concentration (32 mg/L DOC of Fuchskuhle NOM, 38 mg/L DOC of HS 1500) resulted in a decrease in brood size, highlighting an overall intrinsic tolerance toward humic material. Finally, oligonucleotide-based whole genome DNA microarray experiments were performed from control and humic material treated worms. Significant transcriptional changes (exceeding a 2-fold increase or decrease) were identified in chemosensors, olfactory receptors, as well as enzymes of the biotransformation system (cytochromes P450, UDP-glucuronosyltransferases, glutathione S-transferases), thereby confirming that humic material is recognized as an environmental signaling chemical.