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Histopathological and ultrastructural alterations of induced Yersinia enterocolitica infection in mice
Abstract:
Yersinia enterocolitica is an enteric bacterium and infections by this organism are mostly foodborne. It has been implicated to cause enterocolitis, terminal ilitis. diarrhoea, mesenteric lymphadenitis and arthritis in man. Due to paucity of information regarding histopathological and specially ultrastructural alterations in tissues affected, this study was planned with mice as the experimental model. Nine pathogenic Y.enterocoliticaisolates were used to infect 80 albino mice by oral and intraperitoneal route. Pathological alterations were studied by light and electron microscopy. Histopathological examination of intestines showed severe edema, purulent enteritis, goblet cell hyperplasia infiltration of mononuclear cells, thickening of mucosa and necrosis of the tips of villi. Liver showed congestion, hepatocellular degeneration and necrosis, atrophy of hepatocytes and microabcesses. The lungs revealed congestion, edema, haemorrhage and purulent ronchopneumonia, while kidneys showed mild necrotic changes and bacterial emboli in glomeruli. Ultrastructural changes were indicative of mitochondrial degeneration and their loss in kidneys, membranous degeneration with formation of myelin figures in lungs and disorganization, disruption and bleb formation of microvilli in intestines. Y.enterocolitica caused significant histopathological and ultrastructural alterations in experimentally infected mice. Variation in pathogenicity of different strains of Y.enterocolitica was also observed.
Insights
Yersinia enterocolitica infections cause significant histopathological and ultrastructural damage in mice intestines, liver, lungs, and kidneys. Pathogenicity varied among different Yersinia enterocolitica strains, highlighting the need for further research.
Area of Science:
- Microbiology
- Pathology
- Veterinary Medicine
Background:
- Yersinia enterocolitica is a foodborne enteric bacterium causing various human illnesses.
- Information on histopathological and ultrastructural changes in Yersinia enterocolitica infections is limited.
Purpose of the Study:
- To investigate the histopathological and ultrastructural alterations in mice experimentally infected with Yersinia enterocolitica.
- To assess the pathogenicity variations among different Yersinia enterocolitica isolates.
Main Methods:
- Nine pathogenic Yersinia enterocolitica isolates were used to infect 80 albino mice via oral and intraperitoneal routes.
- Pathological alterations were examined using light and electron microscopy.
Main Results:
- Intestinal damage included edema, purulent enteritis, goblet cell hyperplasia, and villi necrosis.
- Liver, lung, and kidney tissues exhibited congestion, degeneration, necrosis, and microabscesses.
- Ultrastructural changes involved mitochondrial damage, membranous degeneration, and microvilli disorganization.
Conclusions:
- Yersinia enterocolitica infection induces substantial histopathological and ultrastructural damage in multiple organs of experimentally infected mice.
- Observed variations in pathogenicity among Yersinia enterocolitica strains warrant further investigation.
