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Transcriptionally active transposable elements in recent hybrid sugarcane
Paula G de Araujo1, Magdalena Rossi, Erika M de Jesus
1Departamento de Botânica, IB-USP, São Paulo, Brazil.
The Plant Journal : for Cell and Molecular Biology
|November 22, 2005
Summary
Transposable elements (TEs) are active in sugarcane genomes, with most TEs expressed in callus tissue. This study reveals widespread TE transcriptional activity in complex plant genomes.
Area of Science:
- Genomics
- Molecular Biology
- Plant Science
Background:
- Transposable elements (TEs) are mobile genetic elements crucial for genome maintenance and evolution.
- Advancements in genome sequencing enable comprehensive analysis of TE impact on plant genomes.
Purpose of the Study:
- To investigate the expression patterns of transposable elements (TEs) in the sugarcane genome.
- To understand the role of TEs in the evolution of complex plant genomes.
Main Methods:
- Analysis of sugarcane transcriptome data to identify TE clones.
- Full cDNA sequencing and classification of TE families.
- Expression analysis using electronic Northerns, macroarray, and transformation experiments across various tissues.
Main Results:
- Identified 267 sugarcane TE clones, assigning 68 to 11 families.
- Callus tissue showed the highest expression of TEs, indicating induction by tissue culture.
- No tissue-specific TE families were found; however, differential expression within families was observed.
- Hopscotch clone-derived U3 regions function as active promoters under transcriptional regulation.
Conclusions:
- Mobile genetic elements are transcriptionally active in the complex sugarcane genome.
- Tissue culture significantly induces TE expression in sugarcane.
- TEs exhibit dynamic expression patterns within the sugarcane genome, contributing to its evolution.