Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Foreign gene expression by a baculovirus vector with an expanded host range.

H Mori1, H Nakazawa, N Shirai

  • 1Department of Applied Biology, Kyoto Institute of Technology, Japan.

The Journal of General Virology
|July 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunohistochemical demonstration of epidermal growth-factor receptors in normal, benign and malignant thyroid tissues.

International journal of oncology·2011
Same author

Reentrant spin-glass transition in a dilute magnet.

Physical review letters·2005
Same author

[Lung cancer with ground glass opacity diagnosed by transbronchial lung biopsy using an ultrathin bronchoscope and virtual bronchoscopy].

Kyobu geka. The Japanese journal of thoracic surgery·2004
Same author

Overexpression of p8 is inversely correlated with apoptosis in pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2001
Same author

Lipomatous change in a brain metastasis from malignant pleural mesothelioma.

Neuroradiology·2001
Same author

Expression of p8 in human pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2001
Same journal

Variability and population structure of watermelon mosaic virus in zucchini crops in Poland.

The Journal of general virology·2026
Same journal

Probing the sequence constraints for the stable incorporation of chimeric NSP1 segments into infectious rotaviruses.

The Journal of general virology·2026
Same journal

Assessment of influenza virus and coronavirus tropism, replication competence and disease severity in <i>ex vivo</i> and <i>in vitro</i> cultures of the human respiratory tract.

The Journal of general virology·2026
Same journal

GRHAL1, a novel lncRNA, regulates HIV-1 gene expression by modulating Tat- and Sp1-mediated HIV-1 LTR activation.

The Journal of general virology·2026
Same journal

Hepatitis B virus X protein induces E6-associated protein-mediated proteasomal degradation of p53 phosphorylated at Ser-15.

The Journal of general virology·2026
Same journal

Sensing the storm: how inflammatory signalling drives reactivation of the human cytomegalovirus major immediate-early promoter.

The Journal of general virology·2026
See all related articles

Researchers engineered a novel nuclear polyhedrosis virus (NPV) by combining Autographa californica multicapsid NPV (AcMNPV) and Bombyx mori NPV (BmNPV). This hybrid virus exhibits a broader host range and successfully expresses firefly luciferase in various insect cells and silkworm larvae.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • Nuclear polyhedrosis viruses (NPVs) are widely used for insecticidal gene expression.
  • Existing NPV systems have limitations in host range and replication efficiency.
  • The BmN and Sf21 cell lines are non-permissive for specific NPVs, posing challenges for genetic manipulation.

Purpose of the Study:

  • To improve NPV-based gene expression systems through DNA recombination.
  • To create a hybrid NPV with a broader host range and enhanced replication capabilities.
  • To develop a recombinant virus for efficient expression of foreign genes in insect systems.

Main Methods:

  • DNA recombination of Autographa californica multicapsid NPV (AcMNPV) and Bombyx mori NPV (BmNPV).

Related Experiment Videos

  • Cotransfection of viral DNA into Sf21 cells followed by plaque purification on BmN cells.
  • Construction of a recombinant virus using the AcMNPV transfer vector pAcYM1 to replace the polyhedrin gene with the firefly luciferase gene.

    • Main Results:

    • Isolation of a hybrid NPV (AcMNPV-BmNPV) with a wider host range, replicating in Sf21 cells, BmN cells, and silkworm larvae.
    • The hybrid virus successfully produced polyhedra in permissive and non-permissive insect cells and larvae.
    • A recombinant virus expressing firefly luciferase under the polyhedrin promoter was generated and demonstrated efficient expression in multiple insect systems.

    Conclusions:

    • DNA recombination is an effective strategy for creating hybrid NPVs with improved characteristics.
    • The developed hybrid and recombinant NPVs offer a versatile platform for gene expression in diverse insect hosts.
    • This improved NPV system holds potential for applications in biotechnology and pest control.