Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A photoactivable probe for calcium binding proteins.

Adrian Israelson1, Laetitia Arzoine, Salah Abu-hamad

  • 1Department of Life Sciences and The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva 84713, Israel.

Chemistry & Biology
|November 22, 2005
PubMed
Summary

Researchers developed azido ruthenium (AzRu), a novel reagent that specifically targets calcium (Ca2+) binding proteins. This tool identifies and inhibits Ca2+-dependent activities, aiding in the discovery of new cellular processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MAVS as a Key Regulator of Tumor Proliferation, Survival, the Tumor Microenvironment, and Immunity.

Biomolecules·2026
Same author

Disrupting VDAC1-tubulin interaction uncovers crosstalk between mitochondrial and microtubule functions with implication to cancer therapy.

Cellular and molecular life sciences : CMLS·2026
Same author

Targeting ST18-mediated pathomechanism in pemphigus vulgaris through voltage-dependent anion channel inhibition.

Skin health and disease·2026
Same author

Impaired nucleocytoplasmic transport in SOD1-mediated ALS.

Molecular neurodegeneration·2026
Same author

Erratum: Survivin/BIRC5-derived peptide disrupts survivin dimerization and cell division and induces multifaceted anti-cancer effects.

Molecular therapy. Oncology·2026
Same author

Survivin/BIRC5-derived peptide disrupts survivin dimerization and cell division and induces multifaceted anti-cancer effects.

Molecular therapy. Oncology·2026

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Calcium ions (Ca2+) are crucial signaling molecules regulating vital cellular processes, including cell life and death.
  • Despite the known importance of Ca2+-dependent activities, many associated proteins remain unidentified.
  • Understanding these proteins is key to deciphering complex cellular signaling pathways.

Purpose of the Study:

  • To develop a novel photoreactive reagent for specific identification and characterization of calcium (Ca2+) binding proteins.
  • To investigate the utility of this reagent in inhibiting Ca2+-dependent activities across various cellular contexts.
  • To provide a new tool for exploring previously unknown Ca2+-dependent biological processes.

Main Methods:

  • Synthesis and characterization of a photoreactive reagent, azido ruthenium (AzRu).

Related Experiment Videos

  • Testing AzRu's specificity for Ca2+ binding proteins and its inhibitory effects on Ca2+-dependent activities.
  • Utilizing AzRu to covalently label known Ca2+ binding proteins in purified, membrane-embedded, and intact cell states.

    • Main Results:

    • AzRu specifically interacts with and strongly inhibits Ca2+ binding proteins, irrespective of their function or state.
    • The reagent demonstrated no impact on Ca2+-independent or Mg2+-dependent activities, confirming its specificity.
    • AzRu successfully labeled known Ca2+ binding proteins, validating its efficacy in protein identification.

    Conclusions:

    • Azido ruthenium (AzRu) is a potent and specific photoreactive reagent for targeting Ca2+ binding proteins.
    • AzRu offers a valuable approach for identifying novel Ca2+ binding proteins and their binding sites.
    • This reagent facilitates the exploration of new Ca2+-dependent cellular functions and signaling pathways.