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Epidemiological markers of Salmonella typhimurium isolates in Rome

A Martini1, E Filetici, M Fantasia

  • 1Laboratorio di Batteriologia e Micologia Medica, Instituto Superiore di Sanità, Rome, Italy.

Journal of Medical Microbiology
|August 1, 1992
PubMed

Insights

This study characterized Salmonella typhimurium strains from Rome using phage typing and DNA plasmid analysis. Phage typing identified epidemiologically linked strains, while plasmid analysis further distinguished strains within the same phage type.

Area of Science:

  • Microbiology
  • Epidemiology
  • Molecular Biology

Background:

  • Salmonella typhimurium is a significant cause of bacterial enteritis.
  • Understanding strain variability is crucial for epidemiological surveillance and control.

Purpose of the Study:

  • To characterize Salmonella typhimurium strains isolated in Rome between 1984-1986.
  • To evaluate the utility of phage typing and DNA plasmid analysis in strain differentiation.

Main Methods:

  • Isolation and characterization of 172 Salmonella typhimurium strains.
  • Determination of phage type, antimicrobial resistance, colicinogenic activity, and DNA plasmid content.
  • Epidemiological data collection from patients and asymptomatic subjects.

Main Results:

  • Most identified phage types were prevalent across multiple years, with some unique to a single year.
  • Phage typing effectively identified epidemiologically related strains.
  • DNA plasmid analysis provided further characterization of strains within the same phage type, particularly during suspected outbreaks.

Conclusions:

  • Phage typing and DNA plasmid analysis are valuable tools for Salmonella typhimurium surveillance.
  • These methods aid in distinguishing between strains and tracking their epidemiological spread.
  • Characterization is essential for understanding outbreaks and implementing targeted control measures.

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