Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification and correction of systematic errors due to detector time-averaging in single-molecule tracking

Nicolas Destainville1, Laurence Salomé

  • 1Laboratoire de Physique Théorique, UMR CNRS-UPS 5152, Université Paul Sabatier, 31062 Toulouse, France.

Biophysical Journal
|November 22, 2005
PubMed
Summary

Single-molecule tracking can overcome detector exposure time limitations. This method analytically corrects for biases in apparent molecular motion, enabling exploration of faster cellular events.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inference of the 3D pressure field exerted by a single cell from a thin membrane transverse deformation.

The European physical journal. E, Soft matter·2026
Same author

Digital One-Step Competitive Detection of a Small Molecule in Synthetic and Environmental Waters.

Analytical chemistry·2024
Same author

There and back again: bridging meso- and nano-scales to understand lipid vesicle patterning.

Soft matter·2024
Same author

LFA-1 nanoclusters integrate TCR stimulation strength to tune T-cell cytotoxic activity.

Nature communications·2024
Same author

Supramolecular organization and dynamics of mannosylated phosphatidylinositol lipids in the mycobacterial plasma membrane.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Protein overexpression can induce the elongation of cell membrane nanodomains.

Biophysical journal·2022

Area of Science:

  • Biophysics
  • Cell Biology
  • Membrane Dynamics

Background:

  • Single-molecule tracking (SMT) is crucial for studying plasma membrane organization.
  • Detector exposure time introduces time-averaging effects, biasing observed molecular motion in microdomains.
  • Numerical simulations suggest these effects limit SMT's accuracy.

Purpose of the Study:

  • To analytically address the limitations imposed by detector exposure time in SMT.
  • To investigate the impact of time-averaging on diffusion coefficients and microdomain size measurements.
  • To develop a method for recovering true molecular parameters from apparent ones.

Main Methods:

  • Analytical solution derived for time-averaging effects in SMT.
  • Calculation of apparent diffusion coefficients and domain sizes under biased conditions.

Related Experiment Videos

  • Comparison of measured apparent parameters with real parameters.

    • Main Results:

    • Demonstrated that analytical methods can correct for time-averaging biases in SMT.
    • Showed that real molecular diffusion parameters and domain sizes can be accurately recovered.
    • Identified that SMT can resolve events occurring faster than the detector exposure time.

    Conclusions:

    • The study provides an analytical framework to overcome detector exposure time limitations in SMT.
    • Accurate characterization of molecular dynamics in plasma membrane microdomains is now feasible.
    • SMT's utility is expanded to investigate rapid cellular processes previously undetectable.