Related Experiment Video
Updated: Aug 14, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids
Jentaie Shiea1, Min-Zon Huang, Hsiu-Jung Hsu
1Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424 Taiwan. jetea@mail.nsysu.edu.tw
Abstract:
A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds.
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Matrix-Assisted Laser Desorption Ionization (MALDI)
MALDI-TOF Mass Spectrometry
Atomic Emission Spectroscopy: Lab
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

