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Updated: Aug 14, 2026

Electrochemiluminescence Assays for Human Islet Autoantibodies
09:15

Electrochemiluminescence Assays for Human Islet Autoantibodies

Published on: March 23, 2018

Immunogenicity testing by electrochemiluminescent detection for antibodies directed against therapeutic human

Michael Moxness1, Suzanna Tatarewicz, Dohan Weeraratne

  • 1Department of Clinical Immunology, Amgen, Inc., Thousand Oaks, CA 91320-1799, USA. mmoxness@amgen.com

Clinical Chemistry
|November 22, 2005
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.