Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Low dose hydroxylated PCB induces c-Jun expression in PC12 cells.

Noriaki Shimokawa1, Wataru Miyazaki, Toshiharu Iwasaki

  • 1Department of Integrative Physiology, Gunma University Graduate School of Medicine, Gunma, Japan. simokawa@med.gunma-u.ac.jp

Neurotoxicology
|November 23, 2005
PubMed
Summary

Polychlorinated biphenyls (PCBs) exposure increases c-Jun expression in neuronal cells. This neurotoxic effect is mediated by sodium and calcium channel activation, not thyroid hormone signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular regucalcin blocks the proliferation and metastatic activity of SK-N-SH human neuroblastoma cells by targeting diverse signaling pathways: Involvement in the cancer cell microenvironment.

Cellular signalling·2026
Same author

Roles of the nuclear receptor corepressor 1 (NCoR1) and the silencing mediator of retinoid and thyroid hormone receptors (SMRT) in the developing brain.

Life sciences·2026
Same author

S-equol Modulates T3-Induced Transcription and Neurite Outgrowth in Neuronal Cells.

International journal of molecular sciences·2026
Same author

Reply to "Medical Education and Artificial Intelligence: Some Suggestions".

JMA journal·2026
Same author

Histological and genetic changes induced by extracorporeal shockwave therapy after rotator cuff repair in a rat model with tears.

Scientific reports·2026
Same author

Use and Evaluation of Generative Artificial Intelligence by Medical Students in Japan.

JMA journal·2025

Area of Science:

  • Environmental toxicology
  • Neuroscience
  • Molecular biology

Background:

  • Polychlorinated biphenyls (PCBs) are environmental pollutants linked to adverse health effects, including neurotoxicity.
  • PCBs can alter gene expression and signal transduction pathways.
  • The role of PCBs in regulating c-Jun expression, a key transcription factor, is not well understood.

Purpose of the Study:

  • To investigate the effect of hydroxylated PCBs (OH-PCBs) on c-Jun expression in PC12 cells.
  • To elucidate the signaling pathways involved in OH-PCB-induced c-Jun expression.

Main Methods:

  • PC12 cells were treated with varying concentrations of OH-PCB.
  • c-Jun expression levels were measured.
  • The involvement of thyroid hormone, c-Jun N-terminal kinases (JNK), and ion channels (calcium and sodium) was assessed using specific blockers.

Related Experiment Videos

Main Results:

  • OH-PCB significantly increased c-Jun expression at low concentrations (10(-8)M and 10(-7)M).
  • The effect was not mediated by thyroid hormone.
  • OH-PCB enhanced JNK phosphorylation.
  • Nimodipine (a Ca2+ channel blocker) partially inhibited OH-PCB-induced c-Jun expression.
  • Tetrodotoxin (a Na+ channel blocker) completely inhibited OH-PCB-induced c-Jun expression.

Conclusions:

  • Hydroxylated PCBs induce c-Jun expression in neuronal cells.
  • The induction of c-Jun expression by OH-PCBs is initiated by plasma membrane depolarization via Na+ influx, followed by Ca2+ influx through voltage-gated calcium channels.
  • These findings highlight a novel mechanism of PCB neurotoxicity involving ion channel modulation and c-Jun activation.