Potentials and pitfalls of DNA array analysis of the endothelial stress response

Endothelial cells respond to inflammatory stimuli with complex genetic alterations that determine the immune response and the outcome of the inflammatory process. An additional layer of complexity is added by the different phenotypes and functional heterogeneity of endothelial cells in the various tissues. To understand these complex gene response patterns and the regulatory pathways involved, many investigators increasingly use DNA microarray analysis. There are, however, many potential pitfalls in the use of microarrays that can result in false data and erroneous conclusions. This review surveys the principles of DNA microarray technology and its applications in endothelial cell research. We also attempt to outline some of the caveats and standard criteria that have to be considered in order to realize the full potential of microarrays in inflammation research.