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Related Experiment Videos

Clock gene expression in the submandibular glands.

M Furukawa1, T Kawamoto, M Noshiro

  • 1Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8553, Japan.

Journal of Dental Research
|November 24, 2005
PubMed
Summary

Salivary glands possess a molecular clock system, with key clock genes exhibiting daily rhythms. This circadian clock influences amylase gene expression in these glands.

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Area of Science:

  • Molecular Biology
  • Chronobiology
  • Physiology

Background:

  • Circadian rhythms are regulated by clock genes in the suprachiasmatic nucleus and peripheral tissues.
  • The molecular basis of circadian regulation in salivary glands remains largely unexplored.

Purpose of the Study:

  • To investigate the presence and function of the molecular clock system in salivary glands.
  • To examine the expression patterns of clock-related and salivary gland-specific genes.

Main Methods:

  • Analysis of clock gene expression (Per1, Per2, Cry1, Bmal1, Dec1, Dec2, Dbp, Reverbalpha) in submandibular glands under light-dark conditions.
  • Assessment of salivary gland-specific genes, including amylase 1 mRNA.
  • Evaluation of gene expression changes in Clock mutant and Cry1/Cry2 knockout mouse models.

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Main Results:

  • Robust circadian expression rhythms were observed for multiple clock genes in submandibular glands.
  • Amylase 1 mRNA levels exhibited a significant circadian rhythm, unlike other examined salivary gland genes.
  • Clock and Cry gene disruptions altered the mRNA levels of clock-related genes and amylase 1.

Conclusions:

  • The findings demonstrate that a Clock- and Cry-dependent molecular clock system is active and functional in salivary glands.
  • This molecular clock influences the circadian expression of specific genes, such as amylase 1, within the salivary glands.