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Related Experiment Videos

Mouse B cell activation is inhibited by CD44 cross-linking.

Tiana L Wyant1, Michael T Fisher, Robert J McKallip

  • 1Virginia Commonwealth University/MCV, Richmond, VA 23298, USA. tlurgoli@hsc.vcu.edu

Immunological Investigations
|November 25, 2005
PubMed
Summary

CD44 cross-linking inhibits murine B cell activation and Ig production via CD40L, particularly IgE. This effect is dependent on immobilized antibodies and specific activation pathways, suggesting CD44

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Lymphocyte activation and trafficking are critical for immune responses.
  • CD44, an adhesion molecule, is vital for T cell activation and trafficking.
  • The role of CD44 in B cell function remains largely unexplored.

Purpose of the Study:

  • To investigate the effects of CD44 cross-linking on murine B cell activation.
  • To determine the impact of CD44 engagement on B cell proliferation and immunoglobulin (Ig) production.
  • To elucidate the role of CD44 in B cell responses mediated by CD40 ligand (CD40L).

Main Methods:

  • Murine B cells were stimulated via CD40L in the presence of anti-CD44 monoclonal antibodies (mAbs) RK3G9 and IM7.
  • Immobilized and soluble anti-CD44 mAbs were used to assess the impact of CD44 cross-linking.

Related Experiment Videos

  • B cell proliferation and Ig production (including IgE) were measured. Activation via LPS and anti-IgM was also examined.

    • Main Results:

    • Immobilized anti-CD44 mAbs significantly inhibited B cell proliferation and Ig production, with a pronounced effect on IgE.
    • Soluble anti-CD44 mAbs did not affect B cell activation.
    • CD44 cross-linking inhibited LPS-induced B cell activation but not anti-IgM-induced activation, suggesting pathway-specific regulation.

    Conclusions:

    • CD44 cross-linking can control polyclonal B cell activation mediated by CD40L.
    • The inhibitory effect is dependent on antibody immobilization and specific activation signals.
    • CD44 plays a regulatory role in B cell activation, with implications for immune responses and B cell-related pathologies.