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Improving high-content-screening assay performance by using division-arrested cells
Chandrasekaran Vasudevan1, Natalie Fursov, Patrick Maunder
1Cellomics Inc., Pittsburgh, PA, USA.
Assay and Drug Development Technologies
|November 25, 2005
Summary
Division-arresting cells using mitomycin C improves consistency in high-content screening assays. This allows for mass production of reliable cell reagents, streamlining the screening process for better drug discovery.
Area of Science:
- Cell biology
- Drug discovery
- Assay development
Background:
- Cell-based assays are increasingly vital for robotic high-throughput compound screening.
- Ensuring consistent and high-quality cell reagents is critical for reliable screening results.
Purpose of the Study:
- To investigate the impact of division-arrested cells on assay consistency.
- To propose a method for decoupling cell production from screening processes.
Main Methods:
- Cells were treated with low-level mitomycin C to induce irreversible division arrest.
- Performance of division-arrested cells was compared to non-arrested cells in high-content screening imaging assays.
Main Results:
- Division-arrested cells demonstrated superior consistency in high-content screening imaging assays compared to non-arrested cells.
- The study successfully validated the use of division-arrested cells as reliable reagents.
Conclusions:
- Uncoupling cell production and screening through division arrest and cryopreservation is feasible.
- "Ready-to-use" division-arrested cell reagents enhance consistency and convenience in cell-based screening.