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Improving high-content-screening assay performance by using division-arrested cells.

Chandrasekaran Vasudevan1, Natalie Fursov, Patrick Maunder

  • 1Cellomics Inc., Pittsburgh, PA, USA.

Assay and Drug Development Technologies
|November 25, 2005
PubMed
Summary
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Division-arresting cells using mitomycin C improves consistency in high-content screening assays. This allows for mass production of reliable cell reagents, streamlining the screening process for better drug discovery.

Area of Science:

  • Cell biology
  • Drug discovery
  • Assay development

Background:

  • Cell-based assays are increasingly vital for robotic high-throughput compound screening.
  • Ensuring consistent and high-quality cell reagents is critical for reliable screening results.

Purpose of the Study:

  • To investigate the impact of division-arrested cells on assay consistency.
  • To propose a method for decoupling cell production from screening processes.

Main Methods:

  • Cells were treated with low-level mitomycin C to induce irreversible division arrest.
  • Performance of division-arrested cells was compared to non-arrested cells in high-content screening imaging assays.

Main Results:

  • Division-arrested cells demonstrated superior consistency in high-content screening imaging assays compared to non-arrested cells.

Related Experiment Videos

  • The study successfully validated the use of division-arrested cells as reliable reagents.

    • Conclusions:

    • Uncoupling cell production and screening through division arrest and cryopreservation is feasible.
    • "Ready-to-use" division-arrested cell reagents enhance consistency and convenience in cell-based screening.