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Updated: Aug 14, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
The mechanism of intein-mediated protein splicing: variations on a theme
Kenneth V Mills1, Francine B Perler
1College of the Holy Cross, Department of Chemistry, 1 College Street, Worcester MA 01610, USA.
Abstract:
Intein-mediated protein splicing is facilitated by four separate but coordinated nucleophilic displacement reactions that result in the excision of the intein and the ligation of the flanking polypeptides, called the exteins. These reactions are catalyzed by the intein plus the first downstream extein amino acid without the assistance of cofactors or auxiliary enzymes. Non-canonical inteins missing conserved nucleophilic residues at the N- or C-terminus likely splice using variations of the standard mechanism.
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