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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides
Richard Kimura1, Julio A Camarero
1Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA.
Abstract:
The present paper reviews the use of expressed protein ligation for the biosynthesis of backbone cyclized polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibility to generate cell-based combinatorial libraries that can be screened inside living cells for their ability to attenuate or inhibit cellular processes.
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