Related Experiment Video
Updated: Jul 24, 2026
12:42
Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia
Published on: December 28, 2014
Expression of interleukin-17 in ischemic brain tissue.
1Department of Neurology, the First Affiliated Clinic College of Harbin Medical University, Harbin, China.
Scandinavian Journal of Immunology
|November 25, 2005
Summary
Interleukin-17 (IL-17) is elevated in human and rat brains after ischemic injury, indicating its role in brain inflammation. Neuroglial cells, not just T cells, may produce IL-17 during ischemic events.
Area of Science:
- Neuroscience
- Immunology
- Pathology
Background:
- Ischemic brain injury triggers acute inflammation and cytokine accumulation.
- Cytokines are crucial for leukocyte recruitment and inflammatory processes in brain injury.
- Interleukin-17 (IL-17) is a recently identified cytokine linked to T-cell activity.
Purpose of the Study:
- To investigate the source and action of IL-17 in cerebral ischemia.
- To compare IL-17 expression in human and rat ischemic brain models.
- To determine the cellular origin of IL-17 in brain ischemia.
Main Methods:
- Immunohistochemistry to assay IL-17 levels in human brain tissue.
- Permanent middle cerebral artery occlusion (pMCAO) in Sprague-Dawley rats.
- In situ hybridization for IL-17 mRNA expression in rats.
- Double-staining for IL-17 and glial fibrillary acidic protein (GFAP) in rat neuroglial cells.
Main Results:
- IL-17 levels were elevated in human ischemic brain hemispheres, peaking at days 3-5 post-ischemia.
- IL-17 mRNA expression increased in rat ischemic hemispheres, peaking around day 6.
- Extensive co-localization of IL-17 and GFAP was observed in rat ischemic hemispheres, suggesting neuroglial cell production.
Conclusions:
- IL-17 is involved in the inflammatory response to human brain ischemia.
- Neuroglial cells are a significant source of IL-17 in the later stages of ischemic brain injury in rats.
- Findings suggest neuroglial cells, in addition to T cells, contribute to IL-17 production during brain ischemia.
Related Concept Videos
01:24T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
01:15Ischemic Stroke ll: Pathophysiology
An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...