Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Classification and identification of bacteria using mass spectrometry-based proteomics.

Jacek P Dworzanski1, A Peter Snyder

  • 1Science Applications International Corporation (SAIC), PO Box 68, Aberdeen Proving Ground, MD 21010-0068, USA. jacek.dworzanski@us.army.mil

Expert Review of Proteomics
|November 26, 2005
PubMed
Summary

Rapid bacterial identification is crucial for public health. Mass spectrometry proteomics offers a fast, accurate method for bacterial classification and phylogenetic analysis using protein sequences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Qualitative, Quantitative and Technological Aspects of the Trichothecene Mycotoxins.

Journal of food protection·2019
Same author

Variable ranking based on the estimated degree of separation for two distributions of data by the length of the receiver operating characteristic curve.

Analytica chimica acta·2015
Same author

Mid-infrared, long wave infrared (4-12 μm) molecular emission signatures from pharmaceuticals using laser-induced breakdown spectroscopy (LIBS).

Applied spectroscopy·2014
Same author

Extracellular protein biomarkers for the characterization of enterohemorrhagic and enteroaggregative Escherichia coli strains.

Journal of microbiological methods·2014
Same author

Standoff photoacoustic detection of explosives using quantum cascade laser and an ultrasensitive microphone.

Applied optics·2013
Same author

Long-wave, infrared laser-induced breakdown (LIBS) spectroscopy emissions from energetic materials.

Applied spectroscopy·2012

Area of Science:

  • Microbiology
  • Proteomics
  • Bioinformatics

Background:

  • Accurate and rapid bacterial identification is essential for public health.
  • Traditional microbiologic methods can be time-consuming.
  • Mass spectrometry (MS) offers a faster alternative for bacterial analysis.

Purpose of the Study:

  • To present mass spectrometry-based proteomics techniques for bacterial classification.
  • To demonstrate the utility of MS in rapidly acquiring taxonomically relevant information.
  • To infer the taxonomic position of bacterial strains using proteomic data.

Main Methods:

  • Utilizing gel-free mass spectrometry proteomics, including matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for fingerprinting.
  • Employing mass analysis of fragments from collision-induced dissociation (CID) for high-throughput peptide sequencing.

Related Experiment Videos

  • Searching experimental peptide sequences against a comprehensive bacterial proteome database.

    • Main Results:

    • Successful assignment of experimental peptide sequences to theoretical bacterial proteomes.
    • Generation of phylogenetic profiles from sequenced peptides.
    • Analysis using numerical taxonomy tools to reveal bacterial relatedness.

    Conclusions:

    • Mass spectrometry proteomics enables rapid and accurate bacterial identification and classification.
    • This approach provides a powerful tool for inferring the taxonomic position of bacterial strains.
    • MS-based methods significantly enhance the speed and efficiency of bacterial analysis for public health applications.