Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[In vivo confocal microscopy in blepharitis].

E M Messmer1, E Torres Suárez, M I Mackert

  • 1Augenklinik der Ludwig-Maximilians-Universität, München.

Klinische Monatsblatter Fur Augenheilkunde
|November 26, 2005
PubMed
Summary

In vivo confocal microscopy reveals meibomian gland dysfunction and inflammation in blepharitis patients. This objective technique aids in classifying and monitoring meibomian gland disease, distinguishing it from meibomitis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Dietary supplements for dry eye disease].

Die Ophthalmologie·2025
Same author

[ChatGPT and the German board examination for ophthalmology: an evaluation].

Die Ophthalmologie·2024
Same author

[Wound healing following amniotic membrane, limbal stem cell and corneal transplantation].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2020
Same author

[Intense pulsed light (IPL) as a therapeutic option for Meibomian gland dysfunction].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2019
Same author

[Retinal detachment in children and adolescents. Specific clinical features].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2019
Same author

[The German keratomycosis registry : Initial results of a multicenter survey].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2019

Area of Science:

  • Ophthalmology
  • Microscopy
  • Cell Biology

Background:

  • Chronic blepharitis is often linked to meibomian gland dysfunction, characterized by inflammation and obstruction.
  • Objective diagnostic tools for direct meibomian gland examination are limited.

Purpose of the Study:

  • To evaluate the utility of in vivo confocal microscopy for assessing meibomian gland pathology in patients with blepharitis.
  • To differentiate between meibomitis and meibomian gland dysfunction using objective imaging.

Main Methods:

  • Nineteen patients with various forms of blepharitis and 10 healthy controls underwent in vivo confocal microscopy (HRTII/RCM).
  • Microscopic scans of the tear film, tarsal conjunctiva, hair follicles, and meibomian glands were analyzed by a masked observer.

Related Experiment Videos

Main Results:

  • Confocal microscopy identified significant ductal dilatation and obstruction in the meibomian glands of patients with blepharitis/meibomitis or meibomian gland dysfunction.
  • Intense inflammation of the tarsal conjunctiva was observed in 15 of 19 blepharitis/meibomitis patients.
  • Meibomian glands appeared normal in patients with anterior blepharitis, autoimmune keratitis, or severe dry eye associated with blepharitis.

Conclusions:

  • In vivo confocal microscopy provides an objective method for visualizing and documenting pathological changes in meibomian gland disease.
  • The presence of inflammatory infiltrates helps distinguish meibomitis from meibomian gland dysfunction.
  • This technique is valuable for the classification and follow-up of blepharitis patients.