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Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
Antitumor effect of double immunization of mice with mucin 1 and its coding DNA
Natalya Mushenkova1, Ekaterina Moiseeva, Alexandra Chaadaeva
1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya St, 16/10, Moscow, 117997, Russian Federation.
Objective:
To evaluate the antitumor effect of mouse immunization with human mucin 1 gene (muc 1) DNA plasmids combined with simultaneous injections of human mucin 1 (MUC1) protein.
Materials And Methods:
MUC1 DNA was cloned in pBK-CMV to prepare DNA plasmids and in pET22b(+) to produce proteins. Three strains of mice, immunized with DNA or DNA plus MUC1, were inoculated with tumor cells obtained from spontaneous tumors. IgG(2a) production, MUC1-specific IFN-delta-producing CD8+ T cells, tumor growth and mouse survival were monitored.
Results:
Only immunization with DNA plus proteins induced IgG(2a) and intracellular IFN-delta production by CD8+ T cells in the strains tested. DNA plus protein immunization induced a better mouse survival in comparison with the DNA groups. However, all immunized mice invariably developed tumors.
Conclusion:
Immunization with DNA plus proteins induced a better protection from tumor growth than immunization with naked DNA. However, the efficacy of immunization with MUC1-based antigens remains low.
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