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Updated: Aug 12, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Understanding the response of dendritic cells to activation by streptococcal preparation OK-432
Norio Kanzaki1, Masanori Terashima, Seigo Kashimura
1Department of Surgery 1, Fukushima Medical University, Fukushima 960-1295, Japan.
Background:
The streptococcal preparation OK-432 induces maturation of T cells and dendritic cells (DCs). However, the mechanisms by which OK-432 induces DC maturation are not well understood.
Materials And Methods:
The effects of OK-432 and TNF-alpha on peripheral blood mononuclear cells (PBMCs) were compared. Antibody-based approaches were used to detect proteins characteristic of antigen-presenting cells and cytokines. Cytotoxicity was evaluated by measuring the release of LDH after incubation of effector and target cells. The TLR-4 levels were measured by real-time quantitative PCR.
Results:
Changes in cell surface marker levels were detected in both treatment groups but OK-432 had a greater effect on induction of Th-1-type cytokines. Furthermore, TLR-4 mRNA was up-regulated in cells from two out of five patients in response to OK-432, but not in response to TNF-alpha.
Conclusion:
OK-432 has a more profound effect on human DCs than TNF-alpha and may act through multiple signaling pathways.
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