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Updated: Aug 14, 2026

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
Published on: February 28, 2020
Molecular changes in PDEGF and bFGF in malignant melanomas in relation to the stromal microenvironment
Michala Fiuraskova1, Svetlana Brychtova, Eva Sedlakova
1Institute of Pathology & Laboratory of Molecular Pathology, Faculty of Medicine, Palacky University, Hnevotinska 3, 77515 Olomouc, Czech Republic. mfiuraskova@seznam.cz
Background:
Aberrant expression of either growth factors or growth factor-receptors by stromal cells can be an important factor promoting the growth of solid tumours. It may also affect differentiation of malignant cells and support tumour spread. The aim of the present study was to investigate the hypothesis that basic-fibroblast growth factor (bFGF) and platelet-derived growth factor (PDEGF) may be involved in tumour-stromal microenvironment interactions in primary malignant melanomas.
Materials And Methods:
PDEGF and bFGF expression in malignant cells and surrounding stromal elements was assessed using indirect immunohistochemistry.
Results:
It was confirmed that PDEGF can be involved in the reciprocal interactions between tumour cells and stroma, including aberrant angiogenesis. Interestingly, bFGF was present both in malignant melanoma lesions and benign nevi accompanied by different intracellular localisation of the protein, suggesting its implication in regulation of nevus cell proliferation and maturation.
Conclusion:
The present results suggest that bFGF and PDEGF participate in malignant melanoma progression.
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