Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimizing plasmid-based gene transfer for investigating skeletal muscle structure and function.

Jonathan D Schertzer1, David R Plant, Gordon S Lynch

  • 1Department of Physiology, The University of Melbourne, Melbourne, VIC 3010, Australia.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|November 29, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Statins promote muscle metabolic danger and NLRP3-mediated myopathy via lower protein-prenylation and YAP.

Science advances·2026
Same author

Postbiotics lower adipose tissue MHCII inflammation and blood glucose in obese mice, dependent on sex.

Physiological reports·2026
Same author

Evaluating skeletal muscle dysfunction and recovery in a zymosan model of critical illness in mice.

Disease models & mechanisms·2026
Same author

Immunometabolism of IRF4 in adipose, muscle, and immune cells influences obesity and MASLD.

American journal of physiology. Endocrinology and metabolism·2026
Same author

Insulin and adipocyte IRF4 promote fat retention over muscle preservation during intermittent fasting in obesity.

Cell reports·2026
Same author

Postbiotic gut bacteria site-specific immunomodulators improve liver steatosis, inflammation, and fibrosis in obese mice with MASLD.

American journal of physiology. Endocrinology and metabolism·2026
Same journal

Targeting of Aberrant αvβ6 Integrin Expression in Solid Tumors Using Chimeric Antigen Receptor-Engineered T Cells.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Neonatal systemic gene therapy restores cardiorespiratory function in a rat model of Pompe disease.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Endothelial injury with capillary leak: A final common pathway in acute AAV toxicity?

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Long-term functional synaptic integration of genome-edited retinal organoids in a primate model of macular degeneration.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Nox1/4 inhibitor Setanaxib treatment ameliorates cardiac function in mouse models of Duchenne Muscular Dystrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

HSP90α-USP7-DNMT1 Axis Drives Hepatocellular Carcinoma Recurrence After Microwave Ablation by Disrupting ACSS3-Mediated Propionate Metabolism.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
See all related articles

This study introduces a novel electroporation method to improve gene delivery to skeletal muscles without causing damage. The optimized protocol enhances transfection efficiency, crucial for developing new therapies for muscle disorders.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Muscle Physiology

Background:

  • Naked plasmid DNA offers a safer alternative to viral vectors for skeletal muscle gene delivery.
  • Low transfection efficiency of plasmid DNA limits its therapeutic and experimental applications.
  • Current methods like electroporation can cause muscle damage, affecting physiological assessments.

Purpose of the Study:

  • To develop and validate an optimized electroporation protocol for enhanced skeletal muscle gene transfer.
  • To ensure the developed protocol minimizes muscle damage and preserves muscle function.
  • To explore the therapeutic potential of this method for muscle-related conditions.

Main Methods:

  • Developed a novel electroporation protocol involving hyaluronidase pretreatment and specific voltage/vehicle parameters.

Related Experiment Videos

  • Administered naked plasmid DNA via intramuscular injection to mouse tibialis anterior muscles.
  • Assessed transfection efficiency using reporter gene expression.
  • Evaluated muscle damage and contractile function at both intact and single-fiber levels.

    • Main Results:

    • The optimized protocol achieved 22 +/- 5% reporter gene expression in muscle fibers without causing significant muscle damage.
    • Electroporation did not compromise the contractile function of skeletal muscles.
    • Ectopic expression of insulin-like growth factor I induced muscle hypertrophy without adverse effects on tissue or function.

    Conclusions:

    • This optimized electroporation protocol significantly enhances gene transfer efficiency in skeletal muscle.
    • The method is non-damaging and preserves muscle function, making it suitable for physiological studies.
    • The approach holds therapeutic promise for treating myopathies and muscle wasting disorders.