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Complement in BuB/BnJ mice revisited: serum C3 levels and complement opsonic activity are not elevated.

Inga Osmers1, Alexander J Szalai, Andrea J Tenner

  • 1Department of Microbiology, 845 19th St. S. BBRB/842, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Molecular Immunology
|November 29, 2005
PubMed
Summary

The BuB/BnJ mouse strain does not exhibit higher complement activity than other inbred strains, contrary to popular belief. Hemolytic assays showed higher activity, but bacterial opsonization assays revealed comparable complement function.

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Area of Science:

  • Immunology
  • Genetics

Background:

  • Inbred mouse strains are crucial models in immunological research.
  • The BuB/BnJ mouse strain is widely believed to possess elevated complement activity and serum protein levels.

Purpose of the Study:

  • To re-examine the accepted views on complement activity in the BuB/BnJ mouse strain.
  • To compare complement serum levels and functional activity across different inbred mouse strains.

Main Methods:

  • Serum C3 levels were measured in BuB/BnJ mice and compared to C57BL/6, Balb/c, and other strains.
  • Bacterial opsonization assays were performed to assess complement's functional activity.
  • Hemolytic assays were conducted to evaluate serum complement activity.

Main Results:

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  • BuB/BnJ mice exhibited serum C3 levels comparable to other common inbred strains.
  • Contrary to expectations, BuB/BnJ mice did not show elevated complement opsonic activity when using bacteria as a substrate.
  • Hemolytic assays, however, indicated that BuB/BnJ sera possessed the highest activity.

Conclusions:

  • The widely held belief of elevated complement activity in BuB/BnJ mice needs re-evaluation.
  • Discrepancies between hemolytic and opsonization assays highlight the complexity of complement function assessment.
  • Further research is warranted to understand the nuances of complement activity in different mouse strains.