Related Experiment Video
Updated: Aug 14, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using
Osamu Morinaga1, Hiroyuki Tanaka, Yukihiro Shoyama
1Innovation Plaza Fukuoka, Japan Science and Technology Agency, Sawara-ku, Fukuoka.
Abstract:
A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol-water-acetic acid (45:55:1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 microg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 microg.
