Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MMTV accessory factor Naf affects cellular gene expression.

Christoph Metzner1, Brian Salmons, Walter H Gunzburg

  • 1Research Institute of Virology and Biomedicine, University of Veterinary Medicine, A-1210 Vienna, Austria. christoph.metzner@vu-wien.ac.at

Virology
|November 29, 2005
PubMed
Summary

Mouse mammary tumour virus (MMTV) negative acting factor (Naf) alters host cell gene expression and growth. Naf

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collecting Eggs, Not Killing Chickens: Why Stem Cell Secretome and Exosomes Are Redefining Regenerative Medicine for Healthspan Extension.

Biomedicines·2026
Same author

Crucial Gram-positive type IV secretion system protein TraF is a structural homolog of type VII secretion system protein EssB/YukC.

microLife·2026
Same author

Computational Modelling of Novelty Detection in the Mismatch Negativity Protocols and Its Impairments in Schizophrenia.

The European journal of neuroscience·2026
Same author

Viral zoonosis and human cancer: further perspectives and considerations.

Infectious agents and cancer·2026
Same author

The IJDD: reflecting on the past and looking to the future.

International journal of developmental disabilities·2026
Same author

Proteomic, Fatty Acid and Mineral Profiles of PDO Arouquesa and Commercial Crossbred Beefs: A Tool for Certification.

Animals : an open access journal from MDPI·2026

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Mouse mammary tumour virus (MMTV) encodes viral superantigen (Sag) and negative acting factor (Naf).
  • Sag and Naf share coding sequences, suggesting potential functional overlap or regulation.
  • Understanding MMTV's impact on host cells is crucial for viral pathogenesis research.

Purpose of the Study:

  • To investigate the functional activities of MMTV's negative acting factor (Naf).
  • To determine if Naf influences host cell protein expression and cellular processes.
  • To differentiate the activities of Naf from those of viral superantigen (Sag).

Main Methods:

  • Utilized 2D-gel electrophoresis (2D-DIGE) to analyze protein expression in Naf-positive cells.
  • Employed luciferase reporter assays to assess gene expression regulation by Naf.

Related Experiment Videos

  • Measured cellular growth rates to evaluate Naf's impact on cell proliferation.

    • Main Results:

    • Identified differential expression of at least 10 cellular proteins in Naf-positive cells.
    • Observed down-regulation of luciferase reporter expression in Naf-expressing cells, linked to decreased cellular growth.
    • Found that dexamethasone induction of Sag did not affect Naf-mediated changes in gene or protein expression.

    Conclusions:

    • Naf exhibits separable activities from Sag, confirming distinct functional roles.
    • Naf plays a role in modulating host cell gene expression during MMTV infection.
    • Naf's influence on cellular growth rates contributes to its overall effect on host cells.