Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydroxyethylstarch microcapsules: a preliminary study for tumor immunotherapy application.

J Devy1, E Balasse, H Kaplan

  • 1Laboratoire de Pharmacotechnie, FRE CNRS 2715, IFR53, Faculté de Pharmacie, 51096 Reims Cedex, France.

International Journal of Pharmaceutics
|November 29, 2005
PubMed
Summary

Hydroxyethylstarch (HES) microcapsules were developed for melanoma immunotherapy, offering protection and slow release of antigens. These biodegradable microcapsules demonstrate non-toxicity and potential for in vivo antigen delivery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Higher blood lipid levels after the transition to menopause in two forager-horticulturalist populations.

Evolution, medicine, and public health·2025
Same author

Deep learning analysis provides accurate COVID-19 diagnosis on chest computed tomography.

European journal of radiology·2020
Same author

Evaluation of mTHPC-loaded PLGA nanoparticles for in vitro photodynamic therapy on C6 glioma cell line.

Photodiagnosis and photodynamic therapy·2019
Same author

Observation of a many-body dynamical phase transition with a 53-qubit quantum simulator.

Nature·2017
Same author

Analysis of the effect of LRP-1 silencing on the invasive potential of cancer cells by nanomechanical probing and adhesion force measurements using atomic force microscopy.

Nanoscale·2016
Same author

Preferred Hearing Aid Response Characteristics Under Acoustic and Telecoil Coupling Conditions.

American journal of audiology·2015

Area of Science:

  • Biomaterials Science
  • Immunology
  • Drug Delivery Systems

Background:

  • Melanoma immunotherapy requires effective antigen delivery systems.
  • Controlled release of antigens is crucial for enhanced immune response.
  • Biodegradable microcapsules offer a promising platform for in vivo applications.

Purpose of the Study:

  • To prepare and characterize hydroxyethylstarch (HES) microcapsules for antigen protection and slow release.
  • To evaluate the loading capacity, entrapment efficiency, and release profile of model protein (BSA).
  • To assess the in vitro and in vivo safety and biodegradability of HES microcapsules.

Main Methods:

  • Interfacial cross-linking of HES with terephthaloyl chloride (TC).
  • Morphological and size characterization using microscopy.

Related Experiment Videos

  • Bovine serum albumin (BSA) loading and release studies.
  • Confocal laser scanning microscopy (CLSM) for visualization.
  • In vitro cytotoxicity assays on melanoma cells.
  • In vivo biodegradability studies after intraperitoneal injection.

    • Main Results:

    • HES microcapsules exhibited sizes in the 4-15 microm range.
    • Maximum loading efficiency of 20% achieved at 2.5% BSA concentration, with near 100% loading capacity.
    • BSA was confirmed to be entrapped within microcapsules.
    • Sustained release of BSA observed, with 80% remaining entrapped for 4 days.
    • Microcapsules showed degradation by alpha-amylase, enhanced by esterase.
    • HES microcapsules were non-toxic to melanoma cells in vitro.
    • Complete biodegradation observed within 7 days after i.p. injection in vivo.

    Conclusions:

    • HES microcapsules are suitable for protecting and controlling the release of antigens.
    • These microcapsules demonstrate excellent loading capacity and sustained release properties.
    • The non-toxicity and in vivo biodegradability of HES microcapsules support their potential as an in vivo drug delivery system for immunotherapy.