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Updated: Aug 14, 2026
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
Published on: May 25, 2018
Distant metastasis from subcutaneously grown E0771 medullary breast adenocarcinoma
Andrew Ewens1, Enrico Mihich, M Jane Ehrke
1Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Background:
Breast cancer treatments are most effective when initiated early, with very poor efficacy against metastatic disease. In seeking a readily metastasizing mouse breast cancer model to facilitate the search for effective therapies, E0771 medullary adenocarcinomas implanted subcutaneously in syngeneic C57BL/6 mice were studied.
Materials And Methods:
Standard pathological, histological and immunological methodologies were used.
Results:
The aggressive estrogen receptor-positive tumor invaded locally into the peritoneal cavity in 56% of mice, as well as metastasizing to the lungs in 52% of mice. The metastasis was a spontaneous event and immunosuppression was seen (e.g. generation of lyphokine activated killer cells and allogeneic cytotoxic T lymphocytes cytolytic activities ex vivo were suppressed). Other pathological events noted as the tumor progressed were: bloody ascites (56%) and shock (72%), both attributed to local (peritoneal) tumor invasion.
Conclusion:
The E0771 metastatic breast cancer model, which mimics the human disease, should be useful in testing new treatments against this disease and/or in examining the metastatic process.
Insights
The E0771 mouse breast cancer model spontaneously metastasizes to the lungs and peritoneal cavity. This model shows immunosuppression and is valuable for testing new breast cancer therapies.
Area of Science:
- Oncology
- Immunology
- Animal Models
Background:
- Effective breast cancer treatment is limited for metastatic disease.
- Early intervention is crucial for successful breast cancer therapy.
- A need exists for a reliable mouse model of breast cancer metastasis.
Purpose of the Study:
- To evaluate the E0771 medullary adenocarcinoma as a model for metastatic breast cancer.
- To characterize the metastatic potential and associated pathologies of the E0771 model in C57BL/6 mice.
Main Methods:
- Subcutaneous implantation of E0771 cells in syngeneic C57BL/6 mice.
- Utilized standard pathological, histological, and immunological techniques for analysis.
Main Results:
- E0771 tumors exhibited aggressive local invasion (56%) and spontaneous lung metastasis (52%).
- Significant immunosuppression was observed, indicated by suppressed immune cell activity.
- Tumor progression led to bloody ascites (56%) and shock (72%) due to peritoneal invasion.
Conclusions:
- The E0771 model effectively mimics human metastatic breast cancer.
- This model is suitable for preclinical testing of novel breast cancer treatments.
- The E0771 model aids in studying the mechanisms of breast cancer metastasis.
