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Tolerosome-induced oral tolerance is MHC dependent
Sofia Ostman1, Maria Taube, Esbjörn Telemo
1Department of Rheumatology and Inflammation Research, Göteborg University, Sweden.
Immunology
|November 30, 2005
Summary
Serum-derived tolerosomes from ovalbumin-fed mice induce antigen-specific tolerance in recipients. This process requires major histocompatibility complex class II expression and is functional only in syngeneic transfers.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Oral administration of protein antigens can induce immune tolerance.
- Serum factors, including tolerosomes expressing MHCII, mediate antigen-specific tolerance.
- The precise function and requirements of serum-derived tolerosomes remain to be fully elucidated.
Purpose of the Study:
- To investigate the in vivo and in vitro functions of serum-derived tolerosomes.
- To determine the role of MHCII expression in tolerosome-mediated oral tolerance.
- To assess the requirements for tolerosome functionality in recipient mice.
Main Methods:
- Purification of tolerosomes from the serum of ovalbumin (OVA)-fed mice.
- In vivo transfer of tolerosomes into naïve and SCID mice.
- In vitro activation assays of OVA-specific T cells.
- Assessment of MHCII expression in intestinal epithelial cells.
Main Results:
- Purified tolerosomes from OVA-fed mice induced OVA-specific tolerance in recipient mice.
- Tolerosomes activated OVA-specific T cells both in vivo and in vitro.
- MHCII expression in intestinal epithelial cells is crucial for tolerosome-induced oral tolerance.
- Tolerosome transfer was only effective in syngeneic recipients, not allogeneic.
Conclusions:
- Tolerosomes can be isolated from mouse serum and mediate antigen-specific oral tolerance.
- Tolerosome-induced oral tolerance is dependent on MHCII expression in intestinal epithelial cells.
- The functional efficacy of tolerosomes is restricted to syngeneic recipients, highlighting the importance of immune compatibility.