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Relationships between circulating matrix metalloproteinase-2 and -9 and renal function in patients with chronic

Horng-Rong Chang1, Shun-Fa Yang, Mei-Lan Li

  • 1Division of Nephrology, Department of Internal Medicine, Chung-Shan Medical University Hospital, Taichung 402, Taiwan.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|November 30, 2005
PubMed
Abstract

Insights

This study found higher matrix metalloproteinase-2 (MMP-2) and lower matrix metalloproteinase-9 (MMP-9) activities in chronic kidney disease (CKD) patients. These enzyme levels correlate with serum creatinine, suggesting a role in CKD pathogenesis.

Area of Science:

  • Nephrology
  • Biochemistry
  • Pathophysiology

Background:

  • Extracellular matrix turnover is implicated in renal fibrosis.
  • Matrix metalloproteinases (MMP-2 and MMP-9) are key enzymes in matrix degradation and renal disease.
  • The relationship between MMP-2, MMP-9, and serum creatinine in chronic kidney disease (CKD) remains uninvestigated.

Purpose of the Study:

  • To investigate the circulating levels of MMP-2 and MMP-9 in patients with CKD.
  • To determine the correlation between MMP-2, MMP-9, and serum creatinine levels in CKD patients.

Main Methods:

  • Plasma samples from 60 CKD patients and 40 controls were analyzed.
  • Gelatin zymography and ELISA were used to quantify MMP-2 and MMP-9 activities.

Main Results:

  • CKD patients exhibited significantly higher serum creatinine and MMP-2 activity (p<0.001).
  • MMP-9 activity and creatinine clearance (CCr) were significantly lower in CKD patients (p<0.05 and p<0.001, respectively).
  • Serum creatinine positively correlated with MMP-2 activity (R=0.288, p<0.05) and inversely with MMP-9 activity (R=0.344, p<0.01).

Conclusions:

  • MMP-2 and MMP-9 levels are altered in CKD patients.
  • A correlation exists between MMP-2, MMP-9, and serum creatinine in CKD.
  • MMP-2 and MMP-9 may play a role in the pathogenesis of CKD.

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