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Updated: Aug 14, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
Published on: May 9, 2014
Reduction of nonspecific binding proteins to self-assembled monolayer on gold surface
Minoru Furuya1, Masayuki Haramura, Akito Tanaka
1Chemistry Department, Reverse Proteomics Research Institute Co. Ltd, 2-6-7 Kazusa-Kamatari, Chiba 292-0818, Japan.
Abstract:
We developed a gold coated glass chip bearing a poly(ethyleneglycol) (PEG) type compound as hydrophilic spacer for surface plasmon resonance studies, which enabled adequate estimation of K(d) value between FK506 and FKBP12 not only using purified FKBP12 (K(d)=22 nM) but also using Escherichia coli lysate expressing FKBP12 (K(d)=15 nM). These results indicated effectiveness of the PEG spacer for reduction of nonspecific interactions. Chemical stability and simple surface-structure of the novel chip are also attractive.

