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Updated: Aug 14, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Published on: October 2, 2018
Separation of caffeine and theophylline in poly(dimethylsiloxane) microchannel electrophoresis with electrochemical
Qian-Li Zhang1, Hong-Zhen Lian, Wei-Han Wang
1The Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Hankou Road 22, Nanjing 210093, China.
Abstract:
A method for the rapid separation and sensitive determination of caffeine and theophylline was presented in poly(dimethylsiloxane) (PDMS) microchannel electrophoresis integrated with electrochemical detection. By using methanol as an additive, the peak shape and resolution were essentially improved. The analytes were well separated within only 40s in the running buffer of 5.0mM borate solution (pH 9.2) containing 10% (v/v) methanol. The linear ranges were from 6microM to 0.6mM and the detection limits were 4microM for caffeine and theophylline, respectively. The proposed method has been successfully applied to determine caffeine and theophylline in rat serum and urine.
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