Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA damage checkpoints in mammals.

Hiroyuki Niida1, Makoto Nakanishi

  • 1Department of Biochemistry and Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.

Mutagenesis
|November 30, 2005
PubMed
Summary

DNA damage triggers cellular responses like repair and cell cycle arrest. This review details how sensor proteins detect damage and signal kinases to halt cell division, preventing mutations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gonocytes in Transition: Establishing the Male Germline Identity.

Advances in experimental medicine and biology·2026
Same author

Preclinical evaluation and mechanistic analysis of antitumor effects of the novel anti-CD147 antibody DS-1471a in liver cancer models.

Investigational new drugs·2026
Same author

Response to Kawamoto et al.

EMBO reports·2026
Same author

Characterization of p16-positive stromal cells in age-related cardiac disorders.

Journal of biochemistry·2026
Same author

DAXX promotes SUMOylation of chromatin-trapped DNMT1.

Journal of biochemistry·2026
Same author

p16<sup>Ink4a</sup>-Positive Hepatocytes Drive Liver Fibrosis Through Activation of LIFR Family Pathway.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DNA damage is a frequent occurrence with potential to cause cancer and aging.
  • Cellular responses to DNA damage include repair, checkpoint activation, and apoptosis.
  • DNA damage checkpoints involve complex signaling pathways that arrest cell-cycle progression.

Purpose of the Study:

  • To review the molecular mechanisms of DNA damage recognition and signal transduction.
  • To classify genes involved in DNA damage checkpoint signaling.

Main Methods:

  • Review of literature on DNA damage response pathways.
  • Analysis of sensor proteins (e.g., 9-1-1 complex, Rad17-RFC complex).
  • Discussion of kinase signaling cascades (ATM, ATR, Chk1, Chk2) and their targets (Cdc25, Wee1, p53).

Main Results:

  • DNA damage is detected by sensor proteins that initiate signaling cascades.
  • Kinases like Chk1 and Chk2 regulate cell cycle inhibitors (Cdc25, Wee1, p53) to arrest cell division.
  • Genes in checkpoint signaling can be categorized into sensors, mediators, transducers, and effectors.

Conclusions:

  • Understanding DNA damage recognition and signaling is crucial for comprehending cancer and aging.
  • The intricate network of sensor proteins and kinases ensures genomic integrity.
  • Classification of checkpoint genes aids in dissecting the complex DNA damage response.

Related Experiment Videos