Related Experiment Video
Updated: Aug 14, 2026
14:12
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Recent applications of microemulsion electrokinetic chromatography
1Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. cwhuie@net1.hkbu.edu.hk
Electrophoresis
|November 30, 2005
Summary
Microemulsion electrokinetic chromatography (MEEKC) offers enhanced separation of hydrophobic compounds and chiral molecules. This technique also aids in determining lipophilicity and partition coefficients for various substances.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Microemulsion electrokinetic chromatography (MEEKC) utilizes oil-in-water or water-in-oil microemulsions for enhanced separation.
- MEEKC offers improved solubilization capacity and migration windows compared to micellar electrokinetic chromatography (MEKC).
Purpose of the Study:
- To review recent applications of MEEKC from 2003-2005.
- To highlight MEEKC's utility in separating chiral molecules and hydrophobic substances.
- To discuss MEEKC's role in determining partition coefficients (lipophilicity).
Main Methods:
- MEEKC employing oil-in-water microemulsions with SDS, octane/heptane, and 1-butanol.
- Reverse MEEKC (water-in-oil microemulsions) for highly hydrophobic compounds.
- Enantioselective MEEKC using chiral surfactants or alcohols for chiral separations.
Main Results:
- MEEKC enables improved separation of hydrophobic and hydrophilic compounds with reduced sample pretreatment.
- Reverse MEEKC enhances the separation of highly hydrophobic substances.
- MEEKC facilitates chiral separations and high-throughput determination of partition coefficients (log P).
Conclusions:
- MEEKC is a versatile technique for separating diverse compounds, including chiral and hydrophobic molecules.
- MEEKC is valuable for characterizing lipophilicity and partition coefficients.
- Recent applications demonstrate MEEKC's broad utility in analyzing pharmaceuticals, cosmetics, biological, environmental, and food samples.
More Related Videos
08:22Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
Published on: February 23, 2020
08:06Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
Published on: February 23, 2017
Related Concept Videos
01:30Capillary Electrophoresis: Applications
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
01:20Electrophoresis: Overview
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
01:09Ion-Exchange Chromatography
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
01:20Capillary Electrophoresis: Instrumentation
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
01:12Electrospray Ionization (ESI) Mass Spectrometry
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
01:06Interfacial Electrochemical Methods: Overview
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...