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HPTLC-densitometric method of determination of oleanolic acid in the Lamii albi flos
Magdalena Wójciak-Kosior1, Tadeusz Krzaczek, Grazyna Matysik
1Laboratory of Planar Chromatography, Department of Chemistry, Medical Academy, Lublin, Poland. kosiorma@wp.pl
Abstract:
HPTLC combined with densitometric method was used to determine the content of oleanolic acid in acetone extract from the Lamii albi flos L. The plant extract was separated on Si 60 HPTLC plates and determined with the use of two densitometric methods: measurement of fluorescence and absorbance. The content of oleanolic acid was statistically calculated. In a measurement of absorbance in 1 g of the dry Lamii albi flos L. 1458 microg of oleanolic acid was determined (0.147%). In a fluorescence method in 1 g of the plant material 1516 microg of oleanolic acid was determined (0.149%).
