Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clonidine increases membrane-associated phospholipase A 2.

U S R Helfrich1, M Schley, G A Petroianu

  • 1University of Heidelberg, Faculty of Clinical Medicine Mannheim, Department of Anaesthesiology and Operative Intensive Care Medicine, Mannheim, Germany.

European Journal of Anaesthesiology
|December 2, 2005
PubMed
Summary

Clonidine significantly increased phospholipase A2 activity in human platelets, contrary to its proposed anti-inflammatory effects. This suggests that clonidine

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nap-dependent declarative memory consolidation in 12-month-old infants - A conceptual replication study.

Infant behavior & development·2026
Same author

Unravelling the Link Between Body Mass Index and Cognitive Performance in Individuals With Bipolar Disorder and Exploration of PRS Moderation Effect: Findings From the PsyCourse Study.

Acta psychiatrica Scandinavica·2025
Same author

The social, psychological, and physical impact of COVID-19 restrictions for institutionalized adults with intellectual and developmental disabilities.

Journal of intellectual disabilities : JOID·2023
Same author

Vinum resinatum: Scientists and unintended consequences.

Die Pharmazie·2021
Same author

Possible metabolic conversion of pinene to ionone.

Die Pharmazie·2020
Same author

[Analysis of a first responder system for emergency medical care in rural areas: first results and experiences].

Der Anaesthesist·2019

Area of Science:

  • Biochemistry
  • Pharmacology
  • Inflammation Research

Background:

  • Alpha2-adrenoreceptor agonists, including clonidine, have been suggested to possess anti-inflammatory properties.
  • Phospholipase A2 (PLA2) is a critical enzyme in synthesizing inflammatory lipid mediators.
  • Understanding PLA2 activity is crucial for elucidating anti-inflammatory mechanisms.

Purpose of the Study:

  • To investigate the effect of clonidine on phospholipase A2 activity.
  • To determine if clonidine's potential anti-inflammatory action is mediated through PLA2 inhibition.

Main Methods:

  • Human platelet membranes with active PLA2 were utilized as an in vitro model.
  • Membranes were exposed to varying concentrations of clonidine or a buffer control.

Related Experiment Videos

  • PLA2 activity was quantified using a radioisotope assay.

    • Main Results:

    • Clonidine exposure resulted in a 4.5-fold increase in PLA2 activity (P < 0.01).
    • Maximal reaction velocity (Vmax) of PLA2 increased significantly post-clonidine.
    • Lineweaver-Burk analysis indicated clonidine interacts with both the PLA2 enzyme and its substrate complex.

    Conclusions:

    • The study concludes that clonidine does not exert anti-inflammatory effects by inhibiting PLA2.
    • Clonidine enhances, rather than inhibits, PLA2 activity in human platelets.