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Updated: Aug 14, 2026
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
Published on: June 23, 2014
[Remodel retinal neovascularization by overexpression of HESR-1 in vitro]
Shi-bo Tang1, Bin Li, Ge Zhang
1Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. sbtang@gzsums.edu.cn
Objective:
To remodel retinal neovascularization by overexpression of HESR-1 in vitro.
Methods:
Human retinal capillary endothelial cell (HRCEC) was isolated, then HRCECs were transfected with pcDNA3.1+HESR-1. The changes of expression of KDR and occluding in HRCECs were analyzed using ELISA assay, the regulation of occluding in HRCECs by HESR-1 was evaluated by western blot.
Results:
Over-expression of HESR-1 down-regulated KDR expression, but occludin expression was up-regulated in HRECEs, the result also showed that the vessels permeability was reduced by HESR-1.
Conclusions:
Overexpression of HESR-1 in HRCECs can inhibit the neovascularization and decrease the permeability of new vessels. It may be a promising gene therapy for intraocular neovascularization.
