Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression profile analysis in astaxanthin-induced Haematococcus pluvialis using a cDNA microarray.

Hyunsuk Eom1, Choul-Gyun Lee, EonSeon Jin

  • 1Department of Biotechnology, Institute of Industrial Biotechnology, Inha University, 253 Yonghyun-Dong, Nam-Gu, 402-751 Incheon, South Korea.

Planta
|December 2, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How ecological lifestyle rewires the architecture of photoprotection across the green lineage.

The New phytologist·2026
Same author

Impact of CP12 deletion on inorganic carbon acquisition and Rubisco partitioning in Chlamydomonas reinhardtii.

Journal of experimental botany·2026
Same author

Mechanical Threshold-Guided Harvesting of Microalgal Therapeutics via Designer Nanovesicles.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Metabolic engineering of Phaeodactylum tricornutum for heterologous production of the plant triterpenoid friedelin.

Microbial cell factories·2025
Same author

Increasing lipid accumulation in Chlamydomonas by serial knocking out of DYRKP1 kinase and ADP-glucose pyrophosphorylase.

Microbial cell factories·2025
Same author

High-yield zeaxanthin production in Chlamydomonas reinhardtii via advanced metabolic pathway engineering.

Biotechnology for biofuels and bioproducts·2025

Haematococcus pluvialis accumulates astaxanthin under stress. Microarray analysis identified 144 differentially expressed genes, including decreased photosynthesis genes and induced defense genes, aiding study of this pigment production.

Area of Science:

  • * Algal biotechnology
  • * Molecular biology
  • * Carotenoid biosynthesis

Background:

  • * The green alga *Haematococcus pluvialis* accumulates the ketocarotenoid astaxanthin under unfavorable conditions.
  • * Astaxanthin accumulation is a stress response, leading to cellular color change from green to red.

Purpose of the Study:

  • * To identify genes differentially expressed during astaxanthin induction in *H. pluvialis* using cDNA microarray analysis.
  • * To validate the reliability of cDNA microarray for monitoring gene expression in *H. pluvialis*.
  • * To identify candidate genes for understanding the molecular mechanisms of astaxanthin accumulation.

Main Methods:

  • * cDNA microarray analysis was performed on *H. pluvialis* cells under high irradiance and nutrient deprivation.

Related Experiment Videos

  • * Gene expression profiles were analyzed for approximately 965 genes.
  • * Real-time PCR was used to validate microarray results.

    • Main Results:

    • * 144 out of 965 genes showed differential expression (twofold change) under astaxanthin-inductive conditions.
    • * Photosynthesis-related genes showed decreased expression in astaxanthin-accumulating (red) cells.
    • * Defense/stress-related genes and signal transduction genes were induced in red cells.

    Conclusions:

    • * cDNA microarray analysis is a reliable method for monitoring gene expression in *H. pluvialis*.
    • * The identified differentially expressed genes provide valuable candidates for further research into astaxanthin biosynthesis.
    • * This study offers tools for dissecting the molecular mechanisms underlying astaxanthin accumulation in *H. pluvialis*.