Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+-surfactant interactions affect enzyme stability in detergent solutions.

Michael R Stoner1, Douglas A Dale, Peter J Gualfetti

  • 1Department of Chemical Engineering, University of Colorado, Boulder, 80309, USA.

Biotechnology Progress
|December 3, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atomic layer deposition for core-shell microparticle vaccines enabling programmable antigen delivery to lymph nodes enhance humoral immune responses.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Single-administration, thermostable human papillomavirus vaccines prepared with atomic layer deposition technology.

NPJ vaccines·2026
Same author

Lipid-free, thermostable mRNA vaccines prepared using atomic layer deposition.

Journal of pharmaceutical sciences·2025
Same author

Broad anti-sarbecovirus responses elicited by a single administration of mosaic-8 RBD-nanoparticle vaccine prepared using atomic layer deposition.

iScience·2025
Same author

Single administration of mosaic-8b RBD-nanoparticle vaccine prepared with atomic layer deposition technology elicits broadly neutralizing anti-sarbecovirus responses.

bioRxiv : the preprint server for biology·2025
Same author

Unsupervised Machine Learning-Based Process Analytical Tools for Near Real-Time Cell Morphology Analysis During CAR-T Cell Manufacturing.

Biotechnology and bioengineering·2025

Detergents can remove essential calcium ions from proteases and amylases, impacting enzyme stability. Adding calcium to detergent formulations can maintain optimal enzyme function by ensuring sufficient free calcium availability.

Area of Science:

  • Biochemistry
  • Enzymology
  • Surfactant Chemistry

Background:

  • Detergent proteases and amylases rely on calcium (Ca2+) ions for stability.
  • Bound Ca2+ ions protect enzymes from degradation, reducing unfolding and proteolysis rates.
  • Surfactant aggregates, like micelles, can indirectly affect enzyme stability by competing for Ca2+.

Purpose of the Study:

  • To investigate the mechanism by which surfactants affect Ca2+ binding to enzymes.
  • To determine the impact of Ca2+-micelle equilibrium on enzyme-Ca2+ interactions.
  • To propose a method for preventing surfactant-mediated Ca2+ removal from enzymes.

Main Methods:

  • Analysis of Ca2+ binding affinities between enzymes and anionic surfactant micelles.
  • Comparison of dissociation constants (Kd) for Ca2+-enzyme and Ca2+-micelle interactions.

Related Experiment Videos

  • Modeling of Ca2+ availability in detergent formulations with varying surfactant and calcium concentrations.

    • Main Results:

    • Surfactant micelles compete with enzymes for Ca2+ ions, with weaker binding affinities (10(-3) to 10(-2) M) compared to enzymes (Kd ~10(-6) M).
    • Despite weaker binding, high surfactant concentrations significantly reduce free Ca2+ available for enzymes.
    • The Ca2+-micelle equilibrium is the primary determinant of free Ca2+ concentration.

    Conclusions:

    • Surfactant-mediated Ca2+ depletion poses a challenge to enzyme stability in detergent formulations.
    • Maintaining a free Ca2+ concentration around 10(-5) M by adding calcium can prevent this issue.
    • Optimizing calcium levels in detergents is crucial for preserving protease and amylase activity.