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Hydrogen production from glucose by anaerobes
Hiroyasu Ogino1, Takashi Miura, Kosaku Ishimi
1Department of Chemical Engineering, Osaka Prefecture University, Sakai, Japan. ogino@chemeng.osakafu-u.ac.jp
Biotechnology Progress
|December 3, 2005
Summary
Clostridium butyricum and Enterobacter aerogenes produced biogas and hydrogen from glucose. Enterobacter aerogenes demonstrated broader substrate utilization for hydrogen production.
Area of Science:
- Microbiology
- Biotechnology
- Anaerobic Fermentation
Background:
- Anaerobic bacteria are crucial for biogas and hydrogen production.
- Glucose is a common substrate for microbial fermentation.
- Understanding substrate utilization is key to optimizing bioprocesses.
Purpose of the Study:
- To investigate hydrogen and biogas production by various anaerobes using glucose.
- To compare the hydrogen production capabilities of Clostridium butyricum and Enterobacter aerogenes.
- To assess the influence of different carbon sources on Enterobacter aerogenes hydrogen production.
Main Methods:
- Cultivation of anaerobic bacteria in thioglycollate medium with glucose.
- Measurement of biogas and hydrogen volume produced.
- Testing of various carbon sources (glucose, pyruvic acid, dextrin, sucrose, maltose, galactose, fructose, mannose, mannitol) for hydrogen production.
Main Results:
- Clostridium butyricum strains produced significant amounts of biogas and hydrogen from glucose.
- Enterobacter aerogenes also produced hydrogen from glucose and pyruvic acid.
- Enterobacter aerogenes exhibited a wider range of substrate utilization, including complex carbohydrates and sugar alcohols, for hydrogen production compared to Clostridium butyricum.
Conclusions:
- Both Clostridium butyricum and Enterobacter aerogenes are capable of producing hydrogen and biogas from glucose.
- Enterobacter aerogenes shows greater versatility in substrate utilization for hydrogen production, making it a promising candidate for bioprocess development.
- Optimizing media composition, such as including yeast extract, can enhance biogas and hydrogen yields.
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