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Updated: Aug 14, 2026

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
Published on: July 17, 2011
Calcium-dependent changes of paired-pulse modulation at single GABAergic synapses
Mykola O Kravchenko1, Anastasia O Moskalyuk, Svetlana A Fedulova
1Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, 4, Bogomoletz str., 01024 Kiev, Ukraine.
Abstract:
Whole-cell patch-clamp and local electrical stimulation were used for measuring of monosynaptic GABAergic currents from rat hippocampal neurons in culture. Under control conditions (normal extracellular calcium, 2 mM) paired-pulse depression with 150 ms interpulse interval was observed. The mean current amplitude for both 1st and 2nd IPSCs displayed bell-shaped dependency from the stimulus amplitude. When extracellular Ca was either decreased to 0.5 mM or increased to 5 mM both mean amplitude of IPSCs and mean release probability decreased/increased correspondingly yet their dependences from the stimulus strength remained bell-shaped. Mean paired-pulse ratio was also affected--we observed facilitation of second IPSC in low-calcium whereas the latter was depressed in high-calcium solution. Our results suggest that calcium concentrations not only regulate the strength of paired-pulse plasticity but also can invert its direction.
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