Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Shigella-type pathomechanism in the "mouse model".

I Kétyi1, J Fischer

  • 1Institute of Microbiology, University Medical School, Pécs, Hungary.

Acta Microbiologica Hungarica
|January 1, 1992
PubMed
Summary

This study shows that Escherichia coli K-12 with a virulence plasmid infects the large bowel in mice, adhering to mucus and penetrating epithelial cells. This leads to significant damage and denudation of the intestinal lining.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fifty years of dysentery research at the Pécs University.

Acta microbiologica et immunologica Hungarica·2002
Same author

A model for testing drug susceptibility of Pseudomonas aeruginosa and Staphylococcus aureus grown in biofilms on medical devices.

Acta microbiologica et immunologica Hungarica·1995
Same author

Biofilms produced by Pseudomonas aeruginosa and by Staphylococcus aureus on model medical devices.

Acta microbiologica et immunologica Hungarica·1995
Same author

Effectiveness of antibiotics on the autochthonous Escherichia coli of mice in the intestinal biofilm versus its planktonic phase.

Acta microbiologica et immunologica Hungarica·1994
Same author

[Effect of antibiotics on autochtonous intestinal E. coli population in SPF mice and on planktonic phase isolates].

Orvosi hetilap·1993
Same author

Rearrangement of upstream regulatory elements leads to ectopic expression of the Drosophila mulleri Adh-2 gene.

Genetics·1992

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Bacterial Pathogenesis

Background:

  • Enteroinvasive Escherichia coli (EIEC) possesses virulence plasmids encoding factors for intestinal colonization.
  • Understanding the role of specific virulence plasmids in E. coli pathogenesis is crucial for developing targeted interventions.

Purpose of the Study:

  • To investigate the virulence and pathogenic mechanisms of Escherichia coli K-12 harboring an enteroinvasive E. coli virulence plasmid (pSP1).
  • To characterize the adherence, invasion, and tissue damage caused by this engineered E. coli strain in a mouse model.

Main Methods:

  • Utilized a mouse model and chick embryos to assess virulence.
  • Examined bacterial adherence to intestinal epithelial surfaces and mucus.
  • Investigated bacterial localization within intestinal crypts and epithelial penetration.

Main Results:

  • The J53(pSP1) E. coli strain demonstrated high virulence in mice and chick embryos, but not in the Serény test.
  • The strain adhered preferentially to goblet cell mucus and showed characteristic intracellular localization within crypt depths.
  • Infection resulted in epithelial surface degeneration and denudation.

Conclusions:

  • The virulence plasmid confers significant pathogenic potential to E. coli K-12, enabling intestinal colonization and tissue damage.
  • The adherence to mucus and invasion into crypts are key steps in the pathogenesis of this EIEC strain.
  • This model provides insights into the mechanisms of enteroinvasive E. coli infections.

Related Experiment Videos